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GATE

Equivalence of Generic Glatiramer Acetate in Multiple Sclerosis: A Randomized Clinical Trial

Year of Publication: 2015

Authors: Jeffrey Cohen, Anna Belova, Krzysztof Selmaj, ..., Frederik Barkhof; for the GATE Study Group

Journal: JAMA Neurology

Citation: JAMA Neurol. 2015;72(12):1433-1441


Clinical Question

Is generic glatiramer acetate (Synthon BV) equivalent to brand glatiramer acetate (Copaxone) in efficacy, safety, and tolerability for the treatment of relapsing-remitting multiple sclerosis?

Bottom Line

Generic glatiramer acetate and brand glatiramer acetate (Copaxone) demonstrated equivalent efficacy as measured by gadolinium-enhancing lesion activity on MRI, with a generic-to-brand ratio of 1.095 (95% CI, 0.883-1.360) fully within the predefined equivalence margin of 0.727-1.375. Safety and tolerability profiles were similar between the two formulations.

Major Points

  • The estimated ratio of generic to brand drug for the primary endpoint (gadolinium-enhancing lesions during months 7-9) was 1.095 (95% CI, 0.883-1.360), fully within the predefined equivalence margin of 0.727-1.375.
  • Study sensitivity was confirmed: combined active treatments had significantly fewer gadolinium-enhancing lesions than placebo (ratio 0.488; 95% CI, 0.365-0.651; P < .001).
  • Per-protocol analysis supported the primary analysis with a generic-to-brand ratio of 1.097 (95% CI, 0.880-1.368).
  • Annualized relapse rates were 0.31 for generic drug, 0.40 for brand drug, and 0.38 for placebo; the trial was not powered to demonstrate relapse rate reduction vs placebo (expected power <30%).
  • Adverse event incidence was similar: 51.0% generic, 54.3% brand, 56.0% placebo. Injection site reactions occurred at similar rates in generic (22.9%) and brand (23.2%) groups.
  • This was the first phase 3 clinical trial of a generic disease-modifying medication for MS.

Design

Study Type: Randomized, multicenter, double-blind, active and placebo-controlled phase 3 equivalence trial

Randomization: 1

Blinding: Double-blind (participants, study personnel, MRI evaluators, steering committee members, and study statistician were unaware of assignments)

Enrollment Period: December 7, 2011 to March 21, 2013

Follow-up Duration: 9 months

Centers: 118

Countries: 17 countries (European Union, North America, and rest of world regions specified)

Sample Size: 794

Analysis: Full analysis set (all randomized participants who received at least 1 injection) and per-protocol set. Primary endpoint analyzed using random-effects generalized linear model with negative binomial distribution and logarithmic link function. Equivalence defined as 95% CI for ratio of generic to brand fully within 0.727-1.375. 92% power to show equivalence, 98% power for study sensitivity, 90% power for both.


Inclusion Criteria

  • Age 18 to 55 years
  • Relapsing-remitting multiple sclerosis fulfilling McDonald criteria
  • Expanded Disability Status Scale (EDSS) score of 0 to 5.5
  • At least 1 documented relapse in the previous year
  • 1 to 15 gadolinium-enhancing lesions on T1-weighted images on screening brain MRI

Exclusion Criteria

  • Clinically significant illness or laboratory abnormalities
  • Prior exposure to brand glatiramer acetate
  • Prior exposure to immunosuppressive treatments
  • Other MS treatments not discontinued for required washout periods (1 to 12 months before screening)

Baseline Characteristics

CharacteristicGeneric Glatiramer Acetate (n=353)Brand Glatiramer Acetate (n=357)Placebo (n=84)
Age, mean (SD)32.6 (8.6)33.8 (9.0)32.6 (8.7)
Age, median (range)32.0 (18.0-56.0)33.0 (18.0-56.0)32.0 (19.0-53.0)
Female sex, No. (%)233 (66.0)238 (66.7)57 (67.9)
Time from onset of symptoms, mean (SD) y5.5 (5.3)6.4 (6.0)5.7 (6.0)
Time from onset of symptoms, median (range) y3.5 (0.1-30.6)4.4 (0.1-30.6)3.3 (0.2-25.7)
No. of relapses in prior 2 y, mean (SD)1.9 (0.9)1.8 (0.9)1.9 (0.9)
No. of relapses in prior 2 y, median (range)2 (1-5)2 (1-6)2 (1-5)
No history of prior disease treatment, No. (%)56 (15.9)62 (17.4)10 (11.9)
EDSS score, mean (SD)2.6 (1.2)2.7 (1.2)2.7 (1.2)
EDSS score, median (range)2.5 (0.0-6.0)2.5 (0.0-5.5)2.5 (0.0-5.5)
Gd-enhancing lesions on T1, mean (SD)2.5 (3.5)2.5 (3.9)2.8 (4.1)
Gd-enhancing lesions on T1, median (range)1 (0-27)1 (0-40)1 (0-28)
T2 hyperintense lesions, mean (SD)51.9 (37.7)49.6 (31.9)47.6 (34.7)
T2 hyperintense lesion volume mm3, mean (SD)9096 (10410)8311 (8386)9122 (11842)
T1 hypointense lesion volume mm3, mean (SD)1686 (3298)1462 (2741)2091 (4329)
Normalized brain volume cm3, mean (SD)1482.0 (85.5)1480.6 (79.7)1482.3 (76.6)

Arms

FieldGeneric Glatiramer AcetateControlControl
InterventionGeneric glatiramer acetate (Synthon BV) 20 mg daily subcutaneous injectionBrand glatiramer acetate (Copaxone) 20 mg daily subcutaneous injectionMatching placebo daily subcutaneous injection
Duration9 months9 months9 months

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Total number of gadolinium-enhancing lesions (cumulative new and persisting) during months 7 through 9 on T1-weighted imagesPrimaryBrand drug: estimated mean 0.41 (95% CI, 0.31-0.54); Placebo: estimated mean 0.82 (95% CI, 0.57-1.20)Generic drug: estimated mean 0.45 (95% CI, 0.34-0.59)P < .001 for combined active treatments vs placebo (study sensitivity); equivalence confirmed by 95% CI within predefined margin
Annualized relapse rateSecondaryBrand: 0.40 (95% CI, 0.26-0.62); Placebo: 0.38 (95% CI, 0.22-0.66)Generic: 0.31 (95% CI, 0.20-0.48)
Patients with no confirmed relapse, No. (%)SecondaryBrand: 264/357 (73.9%); Placebo: 62/84 (73.8%)Generic: 280/353 (79.3%)
Change from baseline in EDSS score, estimated mean (95% CI)SecondaryBrand: -0.08 (-0.19 to 0.03); Placebo: -0.02 (-0.17 to 0.14)Generic: -0.11 (-0.22 to 0.00)
No. of new hyperintense lesions on T2-weighted images, estimated mean (95% CI)SecondaryBrand: 5.9 (3.4-8.4); Placebo: 9.8 (6.4-13.1)Generic: 7.9 (5.4-10.3)
Combined unique active lesions during months 7-9, estimated mean (95% CI)SecondaryBrand: 6.5 (5.1-8.2); Placebo: 10.0 (7.4-13.5)Generic: 8.0 (6.3-10.1)
Change from baseline in T2 hyperintense lesion volume (mm3), estimated mean (95% CI)SecondaryBrand: 358 (-181 to 898); Placebo: 298 (-429 to 1025)Generic: 378 (-161 to 916)
Change from baseline in T1 hypointense lesion volume (mm3), estimated mean (95% CI)SecondaryBrand: 59 (6-113); Placebo: 131 (60-203)Generic: 88 (34-141)
Percent change from baseline in normalized brain volume, estimated mean (95% CI)SecondaryBrand: -0.51 (-0.71 to -0.31); Placebo: -0.56 (-0.82 to -0.29)Generic: -0.48 (-0.68 to -0.28)
Patients disease activity free, No. (%) / estimated % (95% CI)SecondaryBrand: 33/357 (9.2%), estimated 8.5% (4.1-16.5); Placebo: 6/84 (7.1%), estimated 6.6% (2.4-17.1)Generic: 33/353 (9.3%), estimated 8.4% (4.1-16.4)
Per-protocol analysis of primary endpoint (ratio of generic to brand drug)SecondaryRatio: 1.097 (95% CI, 0.880-1.368)
Any adverse eventAdverseBrand: 194/357 (54.3%); Placebo: 47/84 (56.0%)Generic: 180/353 (51.0%)
Any severe eventAdverseBrand: 10/357 (2.8%); Placebo: 0/84 (0%)Generic: 14/353 (4.0%)
Any event leading to discontinuationAdverseBrand: 4/357 (1.1%); Placebo: 2/84 (2.4%)Generic: 12/353 (3.4%)
Any serious adverse eventAdverseBrand: 17/357 (4.8%); Placebo: 2/84 (2.4%)Generic: 12/353 (3.4%)
DeathAdverseBrand: 0; Placebo: 0Generic: 0
Injection site reactionAdverseBrand: 62/357 (17.4%); Placebo: 6/84 (7.1%)Generic: 58/353 (16.4%)
Immediate postinjection reactionAdverseBrand: 18/357 (5.0%); Placebo: 0/84 (0%)Generic: 24/353 (6.8%)
HeadacheAdverseBrand: 12/357 (3.4%); Placebo: 7/84 (8.3%)Generic: 16/353 (4.5%)
Injection site swellingAdverseBrand: 12/357 (3.4%); Placebo: 3/84 (3.6%)Generic: 14/353 (4.0%)
NasopharyngitisAdverseBrand: 23/357 (6.4%); Placebo: 6/84 (7.1%)Generic: 13/353 (3.7%)
Injection site painAdverseBrand: 13/357 (3.6%); Placebo: 1/84 (1.2%)Generic: 11/353 (3.1%)
Local injection site reactions (overall)AdverseBrand: 83/357 (23.2%); Placebo: 14/84 (16.7%)Generic: 81/353 (22.9%)

Subgroup Analysis

Per-protocol set analysis supported the primary analysis with a generic-to-brand ratio of 1.097 (95% CI, 0.880-1.368). Post hoc analysis of only new gadolinium-enhancing lesions (excluding persisting lesions) during months 7-9 also supported the primary analysis.


Criticisms

  • Injection site reactions occurred less often with placebo compared with active treatments, potentially leading to partial unmasking of treatment allocation.
  • The trial was not designed or formally powered to demonstrate relapse rate reduction; with only 84 placebo participants, expected power to demonstrate benefit on relapses was less than 30%, so benefit on relapses relative to placebo was not confirmed.
  • The duration of the trial was short at 9 months, limiting assessment of long-term efficacy, safety, and interchangeability.
  • Formal equivalence margins for other MRI and clinical end points beyond the primary endpoint were not defined.

Funding

Synthon BV

Based on: GATE (JAMA Neurology, 2015)

Authors: Jeffrey Cohen, Anna Belova, Krzysztof Selmaj, ..., Frederik Barkhof; for the GATE Study Group

Citation: JAMA Neurol. 2015;72(12):1433-1441

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