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BRIDGE-TNK ASPECTS

Effect of Baseline ASPECTS on Tenecteplase Efficacy Before Thrombectomy in Acute Large-Vessel Occlusion Stroke: A Post Hoc Analysis of the BRIDGE-TNK Randomized Trial

Year of Publication: 2026

Authors: Huang X, Xu J, Saver JL, et al.

Journal: Neurology

Citation: Neurology 2026;107(6):e218467. DOI: 10.1212/WNL.0000000000218467

Link: https://doi.org/10.1212/WNL.0000000000218467

Bottom Line

IV tenecteplase before thrombectomy significantly improved 90-day functional independence only in patients with ASPECTS <8 (aRR 1.67), with no benefit and numerically higher sICH and mortality in patients with ASPECTS 8–10 (p‑interaction 0.007).

Major Points

  • Post hoc, stratified subgroup analysis of the 550-patient BRIDGE-TNK randomized trial (39 Chinese centers, May 2022–September 2024) using a data-driven ASPECTS cutoff of <8 vs 8–10.
  • ASPECTS <8 (n=241): 90-day mRS 0–2 was 41.6% with tenecteplase+thrombectomy vs 25.0% with thrombectomy alone (aRR 1.67, 95% CI 1.18–2.35, p=0.003); sICH 10.0% vs 11.2%; 90-day mortality 29.2% vs 30.5%.
  • ASPECTS 8–10 (n=309): 90-day mRS 0–2 was 60.6% vs 61.1% (aRR 0.99, 95% CI 0.84–1.17, p=0.92); sICH numerically higher (7.5% vs 2.8%); 90-day mortality 17.6% vs 10.4% (aRR 1.89, 95% CI 0.99–3.61).
  • Significant treatment-by-ASPECTS interactions for the primary outcome (p‑interaction 0.007) and 90-day mortality (p‑interaction 0.04); crossover point for functional independence at ASPECTS 9.
  • Pre-EVT successful reperfusion was numerically higher with tenecteplase in both strata (ASPECTS <8: 6.2% vs 1.6%; ASPECTS 8–10: 6.1% vs 0.7%).
  • Propensity-score-matched analyses (180 in ASPECTS <8, 220 in ASPECTS 8–10) confirmed the primary interaction pattern.
  • Sensitivity analysis restricted to anterior-circulation occlusions with NCCT-based ASPECTS reproduced the interaction (p‑interaction 0.02).
  • In the ASPECTS 0–5 subset (n≈72), tenecteplase yielded numerically higher mRS 0–2 (27.3% vs 11.1%) and significantly higher mRS 0–1 (18.2% vs 2.2%); no patient with ASPECTS 0–2 achieved functional independence.

Design

Study Type: Post hoc subgroup analysis of a randomized controlled trial

Randomization: 1

Blinding: Open-label with blinded outcome assessment (parent trial)

Enrollment Period: May 2022 - September 2024

Follow-up Duration: 90 days

Centers: 39

Countries: China

Sample Size: 550

Analysis: Modified Poisson regression (aRR), proportional odds ordinal regression (cOR), Cox regression (HR) with treatment-by-ASPECTS interaction; 1:1 propensity-score matching; sensitivity analyses


Inclusion Criteria

  • Age eligible for the parent BRIDGE-TNK trial (adult acute ischemic stroke)
  • Acute large-vessel occlusion (anterior or posterior circulation)
  • Eligible for IV thrombolysis
  • Within 4.5 hours of last-known-well time
  • Scheduled to undergo endovascular thrombectomy
  • Written informed consent from patient or legal representative

Exclusion Criteria

  • Contraindication to IV thrombolysis per the parent BRIDGE-TNK protocol
  • Withdrawal of consent (4 of 554 enrolled patients excluded)
  • Standard exclusions for tenecteplase and thrombectomy (parent trial protocol Qiu 2024)
  • Prestroke functional dependence per parent-trial thresholds
  • Any condition precluding 90-day follow-up

Baseline Characteristics

CharacteristicASPECTS <8 - Tenecteplase + Thrombectomy (n=113)ASPECTS <8 - Thrombectomy Alone (n=128)ASPECTS 8-10 - Tenecteplase + Thrombectomy (n=165)ASPECTS 8-10 - Thrombectomy Alone (n=144)
Median Age (IQR), y70 (61-79)69 (62-75)70 (60-77)70 (63-77)
Sex - Male63 (55.8%)73 (57.0%)98 (59.4%)86 (59.7%)
Stratum overall - Median Age (IQR), y69 (61-77)70 (61-77)
Stratum overall - Baseline NIHSS (median)1815
Stratum overall - Glucose (median)7.6 mmol/L7.1 mmol/L
Stratum overall - Internal carotid artery occlusion38.6%23.0%
Stratum overall - MRI-based assessment15.8%8.7%
Stratum overall - Onset-to-randomization (median, min)172153
Stratum overall - Poor collaterals (ASITN/SIR 0-2)80.8%71.2%
Rescue IV tirofiban after thrombectomySignificantly higher than TNK+EVT arm within this stratum

Arms

FieldTenecteplase + ThrombectomyControl
InterventionIV tenecteplase 0.25 mg/kg (max 25 mg) followed by endovascular thrombectomyEndovascular thrombectomy without prior IV thrombolysis
DurationSingle acute treatment; 90-day follow-upSingle acute treatment; 90-day follow-up

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Proportion with functional independence (mRS 0-2) at 90 days, stratified by baseline ASPECTS (<8 vs 8-10) with treatment-by-ASPECTS interactionPrimaryASPECTS <8 - Control (Thrombectomy alone): 32/128 (25.0%) · ASPECTS <8 - Intervention (TNK + Thrombectomy): 47/113 (41.6%) · ASPECTS <8 - aRR (95% CI): 1.67 (1.18-2.35) · ASPECTS <8 - P-value: 0.003 · ASPECTS 8-10 - Control: 88/144 (61.1%) · ASPECTS 8-10 - Intervention: 100/165 (60.6%) · ASPECTS 8-10 - aRR (95% CI): 0.99 (0.84-1.17) · ASPECTS 8-10 - P-value: 0.92 · P-interaction: 0.007
Ordinal shift of 90-day mRS (favorable shift)SecondaryP-interaction: 0.01 · Note: Significant favorable shift with TNK only in ASPECTS <8
mRS 0-1 at 90 d (freedom from disability)SecondaryP-interaction: 0.01
mRS 0-3 at 90 d (ambulatory)SecondaryP-interaction: 0.04
Successful pre-thrombectomy reperfusion (eTICI 2b50/2c/3)SecondaryASPECTS <8 - Control: 1.6% · ASPECTS <8 - Intervention: 6.2% · ASPECTS 8-10 - Control: 0.7% · ASPECTS 8-10 - Intervention: 6.1% · P-interaction: 0.52
Continuous ASPECTS x treatment interaction for 90-day mRS 0-2SecondaryP-interaction: 0.03 · Note: Crossover point at ASPECTS 9
ASPECTS 0-5 subset - mRS 0-2Secondary11.1%27.3%0.07
ASPECTS 0-5 subset - mRS 0-1Secondary2.2%18.2%0.04
Symptomatic ICH (Heidelberg, 48 h) - ASPECTS <8Adverse10.0% (TNK+EVT) vs 11.2% (EVT alone); aRR 0.92 (0.42-1.99)
Symptomatic ICH - ASPECTS 8-10Adverse7.5% vs 2.8%; aRR 2.87 (0.94-8.78); P-interaction 0.11
Any ICH (48 h)AdverseNo significant interaction by ASPECTS (P-interaction 0.53)
90-day mortality - ASPECTS <8Adverse29.2% vs 30.5% (comparable)
90-day mortality - ASPECTS 8-10Adverse17.6% vs 10.4%; aRR 1.89 (0.99-3.61); P-interaction 0.04

Subgroup Analysis

Prespecified interaction by baseline ASPECTS (<8 vs 8-10) showed heterogeneous treatment effect: benefit for functional independence only in ASPECTS <8; possible harm signal (higher sICH and mortality) in ASPECTS 8-10. Sensitivity analysis restricted to anterior-circulation LVO with NCCT-only ASPECTS reproduced the interaction (p-interaction 0.02). Propensity-score-matched analyses (180 in ASPECTS <8, 220 in ASPECTS 8-10) confirmed the primary finding. In the ASPECTS 0-5 exploratory subset, tenecteplase showed favorable outcomes; no ASPECTS 0-2 patient reached functional independence.


Criticisms

  • Post hoc subgroup analysis; the <8 vs 8-10 ASPECTS cutoff was data-driven and not prespecified.
  • Multiple comparisons unadjusted for multiplicity; findings are exploratory and hypothesis-generating.
  • Parent trial was open-label; only outcome adjudication was blinded.
  • Imaging heterogeneity across NCCT, DWI, and pc-ASPECTS may introduce measurement variability despite core-lab review.
  • CT perfusion and quantitative NCCT hypodensity volumetry not routinely available within 4.5 hours, limiting characterization of ischemic core volume.
  • Clot length, a potential IVT-response modifier, was not measured.
  • Enrolled exclusively in China, limiting generalizability to other ethnic populations.
  • Numerically higher sICH and mortality in the ASPECTS 8-10 tenecteplase group raise a safety signal requiring confirmation.

Funding

Anhui Province Clinical Medicine Transformation Special Project (No. 202427b10020048); Key Project of the Department of Education of Anhui Province (No. 2025AHGXZK20227); Research Funds of Centre for Leading Medicine and Advanced Technologies of IHM, China (No. 2023IHM01053). Authors report no relevant disclosures.

Based on: BRIDGE-TNK ASPECTS (Neurology, 2026)

Authors: Huang X, Xu J, Saver JL, et al.

Citation: Neurology 2026;107(6):e218467. DOI: 10.1212/WNL.0000000000218467

Content summarized and formatted by NeuroTrials.ai.