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IST

The International Stroke Trial (IST): a randomised trial of aspirin, subcutaneous heparin, both, or neither among 19 435 patients with acute ischaemic stroke

Year of Publication: 1997

Authors: International Stroke Trial Collaborative Group (writing committee: Sandercock P, Collins R, Counsell C, ..., Warlow C)

Journal: The Lancet

Citation: Lancet. 1997 May 31;349(9065):1569-1581. DOI: 10.1016/S0140-6736(97)04011-7. PMID: 9174558

Link: https://doi.org/10.1016/S0140-6736(97)04011-7

Bottom Line

Early aspirin 300 mg daily started within 48 h of ischaemic stroke onset produced a small but real reduction in death or non-fatal recurrent stroke at 14 days and in death or dependency at 6 months (after adjustment), while subcutaneous heparin conferred no net clinical benefit at 6 months, with medium-dose heparin causing more early bleeding and adverse outcomes than low-dose.

Major Points

  • Large factorial 2×3 trial: 19 435 patients from 467 hospitals in 36 countries randomised within 48 h of suspected acute ischaemic stroke.
  • Aspirin 300 mg daily significantly reduced recurrent ischaemic stroke within 14 days (2·8% vs 3·9%, 2p<0·001) without a significant excess of haemorrhagic stroke.
  • Death or dependency at 6 months with aspirin was 61·2% vs 63·5% (unadjusted 2p=0·06); after adjustment for baseline stroke severity the benefit was significant (14 [SD 6] per 1000, 2p=0·03).
  • Subcutaneous heparin (low + medium combined) yielded no significant reduction in death or dependency at 6 months (62·9% vs 62·9%); fewer recurrent ischaemic strokes were offset by more haemorrhagic strokes.
  • Medium-dose heparin (12 500 IU bd) was more hazardous than low-dose (5000 IU bd), with excess 14-day death or non-fatal stroke (12·6% vs 10·8%, 2p=0·007) and excess transfused/fatal extracranial bleeds (2·0% vs 0·6%).
  • Combined with the concurrently reported Chinese Acute Stroke Trial (CAST, 21 106 patients), aspirin prevented ~10 deaths or recurrent strokes per 1000 during the first few weeks after acute ischaemic stroke.
  • IST supported starting aspirin as soon as ischaemic stroke was suspected once intracranial haemorrhage was excluded, and recommended against subcutaneous heparin regimens more intensive than 5000 IU bd.

Design

Study Type: Randomised controlled trial (open, factorial)

Randomization: 1

Blinding: Open-label treatment; central 6-month outcome assessment blinded to allocation where done

Enrollment Period: January 1991 (pilot) - May 1996 (main trial)

Follow-up Duration: 14 days in hospital and 6 months

Centers: 467

Countries: UK, Italy, Switzerland, Poland, Netherlands, Sweden, Norway, Argentina, Australia, Portugal, Spain, New Zealand, Czech Republic, Turkey, Belgium, Austria, India, Greece, Singapore, Hong Kong, USA, Canada, Israel, Hungary, Brazil, Slovak Republic, Slovenia, South Africa, Chile, Denmark, Ireland, Finland, Japan, Romania, Sri Lanka, France

Sample Size: 19435

Analysis: Intention-to-treat (patients irrevocably included at randomisation); central minimisation for baseline prognostic factors; outcome data 99·99% complete at 14 days and 99·2% at 6 months


Inclusion Criteria

  • Evidence of acute stroke of any severity in the view of the responsible physician
  • Symptom onset less than 48 hours previously
  • No evidence of intracranial haemorrhage (CT before randomisation preferred; mandatory in comatose patients)
  • No clear indication for, or contraindication to, heparin or aspirin
  • Physician genuinely uncertain whether to administer either or both trial treatments (uncertainty principle)
  • Informed consent obtained per local ethics-committee requirements

Exclusion Criteria

  • Only small likelihood of worthwhile benefit (e.g., symptoms likely to resolve completely within a few hours)
  • Severely disabled before the stroke
  • Hypersensitivity to aspirin
  • Active peptic ulceration or recent gastrointestinal bleeding
  • Already on long-term oral anticoagulants
  • Any other clear contraindication to heparin or aspirin

Baseline Characteristics

All (n=19,435):

  • Age <50 yr: 986 (5%)
  • Age 50-59 yr: 2029 (11%)
  • Age 60-69 yr: 4487 (23%)
  • Age 70-79 yr: 6808 (35%)
  • Age >=80 yr: 5125 (26%)
  • Sex - Female: 9028 (46%)
  • Sex - Male: 10407 (54%)
  • Onset while awake: 13750 (71%)
  • Onset during sleep: 5685 (29%)
  • Conscious - Alert: 14921 (77%)
  • Conscious - Drowsy: 4254 (22%)
  • Conscious - Unconscious: 260 (1%)
  • Cardiac rhythm - Sinus (not AF): 16266 (84%)
  • Cardiac rhythm - Atrial fibrillation: 3169 (16%)
  • Systolic BP <140 mmHg: 3590 (18%)
  • Systolic BP 140-159 mmHg: 5402 (28%)
  • Systolic BP 160-179 mmHg: 5035 (26%)
  • Systolic BP >=180 mmHg: 5408 (28%)
  • Stroke syndrome - Total anterior: 4638 (24%)
  • Stroke syndrome - Partial anterior: 7912 (40%)
  • Stroke syndrome - Posterior circulation: 2228 (12%)
  • Stroke syndrome - Lacunar: 4657 (24%)
  • Leg weakness present: 14678 (76%)
  • CT before randomisation: 13020 (67%)
  • CT after randomisation: 5569 (29%)
  • No CT done/known: 846 (4%)
  • Infarct visible on pre-rand CT: 6415 of 13020 (49%)
  • Delay from symptoms 0-3 h: 843 (4%)
  • Delay 4-6 h: 2322 (12%)
  • Delay 7-12 h: 4114 (21%)
  • Delay 13-24 h: 5568 (29%)
  • Delay 25-48 h: 6588 (34%)
  • Aspirin within previous 3 days: 3940 (20%)
  • Heparin within previous 24 h: 436 (2%)

Arms

FieldAspirin 300 mg dailyControlLow-dose subcutaneous heparinMedium-dose subcutaneous heparinControl
InterventionOral aspirin 300 mg once daily (via NG tube, 300 mg suppository, or 100 mg IV lysine salt if unable to swallow) for up to 14 days or until dischargeNo aspirin or other antiplatelet therapy during the first 14 daysUnfractionated heparin 5000 IU subcutaneously twice daily for up to 14 days (~half of heparin arm)Unfractionated heparin 12 500 IU subcutaneously twice daily for up to 14 days (~half of heparin arm)No heparin during the first 14 days
DurationUp to 14 daysUp to 14 daysUp to 14 daysUp to 14 daysUp to 14 days

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Death from any cause within 14 days and death or dependency (needing help from another person with daily activities) at 6 monthsPrimaryaspirin 6-mo adjusted 2p=0·03; heparin 6-mo NS
Recurrent ischaemic stroke within 14 d (aspirin vs no aspirin)Secondary378/9715 (3·9%)275/9720 (2·8%)2p<0·001
Recurrent ischaemic stroke within 14 d (heparin vs no heparin)Secondary370/9718 (3·8%)283/9717 (2·9%)2p=0·005
Symptomatic haemorrhagic stroke within 14 d (aspirin vs no aspirin)Secondary74 (0·8%)87 (0·9%)NS
Symptomatic haemorrhagic stroke within 14 d (heparin vs no heparin)Secondary41 (0·4%)120 (1·2%)2p<0·00001
Death or non-fatal recurrent stroke within 14 d (aspirin)Secondary1208 (12·4%)1099 (11·3%)2p=0·02
Death or non-fatal recurrent stroke within 14 d (heparin)Secondary1171 (12·0%)1136 (11·7%)NS
Pulmonary embolism within 14 d (aspirin)Secondary77 (0·8%)57 (0·6%)2p=0·08 (NS)
Pulmonary embolism within 14 d (heparin)Secondary81 (0·8%)53 (0·5%)2p=0·02
Death from any cause at 6 mo (aspirin)Secondary2168 (22·5%)2073 (21·5%)NS
Death from any cause at 6 mo (heparin)Secondary2076 (21·5%)2165 (22·5%)NS
Medium-dose (12 500 IU bd) vs low-dose (5000 IU bd) heparin: death or non-fatal stroke at 14 dSecondary526/4860 (10·8%)610/4856 (12·6%)2p=0·007
Medium vs low-dose heparin: transfused/fatal extracranial haemorrhageSecondary30 (0·6%)99 (2·0%)2p<0·00001
Transfused or fatal extracranial haemorrhage (aspirin vs no aspirin)Adverse109 (1·1%) vs 57 (0·6%); +5 (SD 1) per 1000; 2p=0·0004
Transfused or fatal extracranial haemorrhage (heparin vs no heparin)Adverse129 (1·3%) vs 37 (0·4%); +9 (SD 1) per 1000; 2p<0·00001
Deaths attributed to haemorrhagic stroke (heparin vs no heparin)Adverse28 vs 15; 2p=0·04
Deaths attributed to extracranial bleeding (heparin vs no heparin)Adverse12 vs 3; 2p=0·02
Aspirin without heparin - transfused/fatal extracranial haemorrhageAdverseexcess 2 (SD 1) per 1000; NS
Medium-dose heparin - haemorrhagic stroke (fatal or non-fatal)Adverse1·8% (medium) vs 0·7% (low); +10 (SD 2) per 1000

Subgroup Analysis

Effects were broadly consistent across prespecified subgroups defined by age, sex, delay from onset (0-3, 4-6, 7-12, 13-24, 25-48 h), consciousness, systolic BP, stroke syndrome, CT timing, and predicted risk. Among patients in atrial fibrillation, heparin reduced recurrent ischaemic stroke by 21/1000 (2·8% vs 4·9%) but caused 16 extra haemorrhagic strokes per 1000, yielding no net benefit. The combination of low-dose subcutaneous heparin + aspirin looked more favourable than aspirin alone at 14 days (early death 8·0% vs 9·3%; early recurrent stroke or ICH 2·8% vs 3·7%) but this was based on ~6000 patients and was not confirmed at 6 months.


Criticisms

  • Open-label treatment without placebo control; potential for ascertainment bias in unblinded in-hospital events, though central 6-month follow-up was largely blinded.
  • Pulmonary embolism appeared under-reported (0·7% overall vs 3-39% in published reviews), limiting inferences about heparin's VTE benefit.
  • Broad eligibility (uncertainty principle) with limited pre-randomisation CT in 33%, and rapid administrative diagnosis instead of a formal severity score (e.g., NIHSS).
  • Fixed subcutaneous heparin dosing without routine APTT monitoring; no intravenous heparin arm.
  • Wide 48-h enrolment window with a median delay of 19 h means most patients were treated well beyond the early hyperacute period, limiting inference for very-early treatment.
  • 6-month outcome collected largely by mailed questionnaire or phone in most countries (using the validated Warlow simple questions).

Funding

UK Medical Research Council; UK Stroke Association; European Union BIOMED-1 programme. Additional support for collaborators' meetings/travel from Eli Lilly, Sterling Winthrop (now Bayer USA), Sanofi, and Bayer UK. Country-specific grants from the National Heart Foundation of Australia, Nova Scotia Heart & Stroke Foundation (Canada), IGA Czech Ministry of Health, McMaster INCLEN and All India Institute of Medical Sciences, Julius Brendel Trust and Lottery Grants Board (NZ), Norwegian Council on Cardiovascular Disease and Nycomed (insurance).

Based on: IST (The Lancet, 1997)

Authors: International Stroke Trial Collaborative Group (writing committee: Sandercock P, Collins R, Counsell C, ..., Warlow C)

Citation: Lancet. 1997 May 31;349(9065):1569-1581. DOI: 10.1016/S0140-6736(97)04011-7. PMID: 9174558

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