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ReHAND-BCI

The ReHand-BCI trial: a randomized controlled trial of a brain-computer interface for upper extremity stroke neurorehabilitation

Year of Publication: 2025

Authors: Jessica Cantillo-Negrete, Martin Emiliano Rodriguez-Garcia, Paul Carrillo-Mora, ..., Ruben I. Carino-Escobar

Journal: Frontiers in Neuroscience

Citation: Cantillo-Negrete J, Rodriguez-Garcia ME, Carrillo-Mora P, Arias-Carrion O, Ortega-Robles E, Galicia-Alvarado MA, Valdes-Cristerna R, Ramirez-Nava AG, Hernandez-Arenas C, Quinzanos-Fresnedo J, Pacheco-Gallegos MdR, Marin-Arriaga N and Carino-Escobar RI (2025) The ReHand-BCI trial: a randomized controlled trial of a brain-computer interface for upper extremity stroke neurorehabilitation. Front. Neurosci. 19:1579988. doi: 10.3389/fnins.2025.1579988

Link: https://doi.org/10.3389/fnins.2025.1579988


Clinical Question

Does a BCI intervention using the ReHand-BCI system with robotic hand orthosis feedback produce greater upper extremity motor recovery and neuroplastic changes compared to a sham-BCI intervention in stroke patients with hand paresis?

Bottom Line

Both BCI and sham-BCI groups showed clinically meaningful improvements in upper extremity sensorimotor function (FMA-UE and ARAT), but no statistically significant intergroup differences were observed. The experimental group showed non-significant trends toward greater ipsilesional cortical activity, higher corticospinal tract integrity, and more lasting corticospinal excitability, suggesting potential neuroplasticity advantages of closed-loop BCI feedback that the study was underpowered to detect.

Major Points

  • Both groups demonstrated significant intragroup improvements in FMA-UE from baseline to post-treatment and follow-up, with changes within the range of the minimal clinically important difference (5.25 points)
  • Only the experimental group showed significant ARAT improvement at post-treatment (baseline 8.5 to post-treatment 20), while the control group achieved significance only at follow-up (baseline 3 to follow-up 15)
  • No statistically significant intergroup differences were found for any primary or secondary outcome at any timepoint
  • The experimental group showed trends toward more pronounced ipsilesional cortical activity measured by alpha LC during affected hand motor intention, and higher ipsilesional corticospinal tract integrity measured by rFA
  • BCI classification accuracy was similar between groups (experimental 67.3% +/- 8.97% vs. control perceived 69.28% +/- 4.1%, p=0.557), indicating comparable engagement with the system
  • Target sample size of 30 was not reached due to conclusion of study funding; only 19 patients were analyzed, making the study likely underpowered to detect intergroup differences
  • This is the first controlled clinical trial to assess such a wide range of physiological effects (EEG, fMRI, DTI, TMS) across a long BCI intervention for stroke rehabilitation

Design

Study Type: Randomized Controlled Trial

Randomization: 1

Blinding: Triple-blinded (patients, clinicians performing outcome assessments, personnel performing statistical analyses)

Enrollment Period: March 16, 2021 to January 16, 2024

Follow-up Duration: 6 months after baseline measurement (T3)

Centers: 1

Countries: Mexico

Sample Size: 23

Analysis: Non-parametric statistics (Mann-Whitney U tests for intergroup, Friedman tests with Bonferroni correction for intragroup); Pearson's correlation coefficient for effect size of intergroup comparisons; Kendall's W for intragroup effect size; Fisher's exact test for sex and lesioned hemisphere; two-sample t-test for BCI classification accuracy; 95% confidence level; SPSS version 30


Inclusion Criteria

  • Adults >18 years
  • Any sex
  • First ischemic or hemorrhagic stroke in either hemisphere with diagnosis confirmation through neuroimaging
  • Time since stroke onset 3 to 24 months
  • Diagnosed hand paresis with Motricity index 0 to 22
  • Right-handed before stroke (to prevent cortical activity variability due to hand dominance)
  • Normal or corrected to normal vision
  • At most mild alterations in attention and memory per NEUROPSI neuropsychological test
  • No previous diagnosed neurological diseases

Exclusion Criteria

  • Clinical diagnosis of severe hand spasticity (Modified Ashworth Scale > 2)
  • Severe aphasia
  • Severe depression
  • Severe attention deficits
  • Previous diagnosis of traumatic brain injury, spinal cord injury, or peripheral nerve injury
  • Having a pacemaker or metal implants incompatible with magnetic resonance
  • Prescriptions of medicaments that interfere with recovery or cognitive performance

Arms

FieldExperimental Group (EG)Control
Intervention30 sessions of BCI therapy using the ReHand-BCI system: EEG-based brain-computer interface decoding motor intention of the paralyzed hand to control a robotic hand orthosis providing passive finger flexion at 25% of maximum displacement when motor intention was classified30 sessions of sham-BCI therapy using the ReHand-BCI system in Sham mode: patients attempted motor intention but the robotic hand orthosis activated randomly (55-85% probability) independent of the system's recognition of hand motor intention
Duration6 weeks (5 sessions per week, 30 sessions total; 80 trials per session in 4 runs of 20 trials)6 weeks (5 sessions per week, 30 sessions total; 80 trials per session in 4 runs of 20 trials)

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Fugl-Meyer Assessment for the Upper Extremity (FMA-UE) and Action Research Arm Test (ARAT) measured at T0 (baseline), T1 (after 15 sessions), T2 (end of 30 sessions), and T3 (6 months post-baseline)PrimaryFMA-UE: T0 median 26 [16,37.5], T2 median 34 [17.3,46.5]; ARAT: T0 median 3 [1.8,30.5], T3 median 15 [2.5,40.8]FMA-UE: T0 median 24.5 [20,36], T2 median 28 [23,43]; ARAT: T0 median 8.5 [5,26], T2 median 20 [7,36]FMA-UE intergroup: T0 p=0.656, T1 p=1, T2 p=0.656, T3 p=0.656; ARAT intergroup: T0 p=1, T1 p=1, T2 p=1, T3 p=0.656 (all non-significant)
Laterality Coefficient (LC) from EEG - alpha band during affected hand motor intentionSecondaryNo significant intragroup changes (p=0.316)No significant intragroup changes (p=0.516, W=0.076)Intergroup: T0 p=0.656, T1 p=1, T2 p=0.327, T3 p=0.656
Laterality Coefficient (LC) from EEG - alpha band during unaffected hand movementSecondaryNo significant intragroup changes (p=0.706)No significant intragroup changes (p=0.373, W=0.104)Intergroup: T0 p=0.37, T1 p=0.37, T2 p=1, T3 p=1
Laterality Coefficient (LC) from EEG - beta band during affected hand motor intentionSecondaryNo significant intragroup changes (p=0.269)No significant intragroup changes (p=0.696, W=0.048)Intergroup: T0 p=0.37, T1 p=0.656, T2 p=0.37, T3 p=1
Laterality Coefficient (LC) from EEG - beta band during unaffected hand movementSecondaryNo significant intragroup changes (p=0.129)No significant intragroup changes (p=0.118, W=0.196)Intergroup: T0 p=0.07, T1 p=1, T2 p=0.37, T3 p=1
Laterality Index (LI) from fMRI - affected hand motor intentionSecondaryNo significant intragroup changes (p=0.769)No significant intragroup changes (p=0.179, W=0.516)Intergroup: T0 p=0.656, T1 p=0.179, T2 p=0.656, T3 p=0.656
Laterality Index (LI) from fMRI - unaffected hand movementSecondaryNo significant intragroup changes (p=0.506)No significant intragroup changes (p=0.656, W=0.392)Intergroup: T0 p=0.179, T1 p=0.179, T2 p=0.656, T3 p=0.656
Ratio of Fractional Anisotropy (rFA) from DTI - corticospinal tract integritySecondaryNo significant intragroup changes (p=0.068, W=0.264)Significant intragroup difference (p=0.045, W=0.268) but post-hoc tests not significant after Bonferroni correctionIntergroup: T0 p=0.179, T1 p=0.179, T2 p=0.656, T3 p=0.179
BCI Classification Accuracy across 30 therapy sessionsSecondaryPerceived accuracy 69.28% +/- 4.1%67.3% +/- 8.97%p=0.557, d=-0.263
OverallAdverseNo adverse effects reportedNo adverse effects reported; 2 patients excluded from analysis due to technical issues with MRI resonator

Subgroup Analysis

No formal subgroup analyses were performed. Descriptive analysis of MEP amplitudes was done separately for ipsilesional and contralesional hemispheres at 100%, 120%, and 140% of resting motor threshold. At T0, 5 EG and 6 CG patients had measurable ipsilesional MEPs. At T2, 5 EG and 6 CG had measurable ipsilesional MEPs. At T3, 4 EG and 4 CG had measurable ipsilesional MEPs. The EG showed higher MEP amplitudes at 120% and 140% RMT in the ipsilesional hemisphere at T2 and T3 compared to CG.


Criticisms

  • Target sample size of 30 patients was not reached (only 23 enrolled, 19 analyzed) due to conclusion of study funding, making the study underpowered to detect intergroup differences
  • Heterogeneity of stroke lesions among participants (cortical, subcortical, cortical-subcortical) may have contributed to variability in recovery profiles
  • Most participants were in the chronic stage with some in late subacute stage, introducing variability from different stroke etiologies and stages following distinct recovery trajectories
  • No significant intergroup differences were found for any primary or secondary outcome, so the primary hypothesis that the experimental group would have higher recovery was not confirmed
  • The sham-BCI intervention provided similar robotic feedback (random activation 55-85%) which may have elicited similar therapeutic effects as actual BCI, making differentiation difficult
  • Conventional therapy was not restricted between T2 and T3 follow-up period, potentially confounding long-term outcome comparisons
  • MEPs could not be recorded in all patients due to severely affected corticospinal tracts, reducing the TMS analysis sample further

Funding

Consejo Nacional de Humanidades, Ciencias y Tecnologias (CONAHCYT) [grant number SALUD-2018-02-B-S-45803]

Based on: ReHAND-BCI (Frontiers in Neuroscience, 2025)

Authors: Jessica Cantillo-Negrete, Martin Emiliano Rodriguez-Garcia, Paul Carrillo-Mora, ..., Ruben I. Carino-Escobar

Citation: Cantillo-Negrete J, Rodriguez-Garcia ME, Carrillo-Mora P, Arias-Carrion O, Ortega-Robles E, Galicia-Alvarado MA, Valdes-Cristerna R, Ramirez-Nava AG, Hernandez-Arenas C, Quinzanos-Fresnedo J, Pacheco-Gallegos MdR, Marin-Arriaga N and Carino-Escobar RI (2025) The ReHand-BCI trial: a randomized controlled trial of a brain-computer interface for upper extremity stroke neurorehabilitation. Front. Neurosci. 19:1579988. doi: 10.3389/fnins.2025.1579988

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