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Vamorolone for DMD

Vamorolone Safety, Pharmacokinetics, and Exploratory Efficacy in Duchenne Muscular Dystrophy: A Phase II, Nonrandomized, Multiple-Dose Study in 2-<4-Year-Old Boys

Year of Publication: 2026

Authors: Mah JK, Gonorazky HD, Nigro E, et al.

Journal: Neurology

Citation: Neurology 2026;106(11):e218066

Link: https://doi.org/10.1212/WNL.0000000000218066

Bottom Line

In 20 corticosteroid-naive boys aged 2-<4 years with DMD, 12 weeks of vamorolone (2 or 6 mg/kg/d) was well tolerated with no serious adverse events and stable growth, dose-dependent adrenal suppression (asymptomatic), dose-dependent pharmacokinetics, and dose-dependent gross motor improvement favoring 6 mg/kg/d.

Major Points

  • Phase II, open-label, nonrandomized, multiple-dose study at 5 Canadian sites; 10 patients each received vamorolone 2 mg/kg/d then 6 mg/kg/d sequentially for 12 weeks, followed by an Expanded Access Protocol (EAP) with data snapshot at ~2 years of exposure.
  • Safety: 70% (2 mg/kg/d) and 90% (6 mg/kg/d) had at least one TEAE; no deaths, no serious TEAEs, no discontinuations; most TEAEs were mild GI events (80% at 6 mg/kg/d) and infections (50% at 2 mg/kg/d, mainly nasopharyngitis).
  • Adrenal suppression (morning cortisol ≤65 nmol/L) at week 12 occurred in 20% (2/10) at 2 mg/kg/d and 40% (4/10) at 6 mg/kg/d; all asymptomatic; 3 patients required hydrocortisone stress dosing during study.
  • Growth: stable height, weight, and BMI z-scores over 12 weeks; ~2 years of EAP follow-up showed median CBL height-score 0.066, BMI-score 0.234; 8/19 patients had BMI-score change >0.5 suggesting excessive weight gain.
  • Pharmacokinetics: dose-dependent Cmax and AUC with rapid absorption (Tmax ~2 h); no accumulation between day 1 and week 2; profile consistent with prior data in 4-<7 year-olds.
  • Exploratory efficacy: median Bayley-III gross motor scaled score change from baseline to week 12 was 0.0 (2 mg/kg/d) vs 2.5 (6 mg/kg/d), suggesting dose-dependent motor benefit favoring 6 mg/kg/d.
  • Bone turnover biomarkers (osteocalcin, CTX1, P1NP) and glucose metabolism markers (fasting glucose, HbA1c, insulin) remained stable at both doses.
  • Class IV evidence given the small (n=20), open-label, nonrandomized design; no formal hypothesis testing was planned.

Design

Study Type: Phase II, open-label, nonrandomized, multiple-dose clinical trial

Randomization:

Blinding: Open-label

Enrollment Period: March 21, 2022 - June 17, 2024 (last patient completed VBP15-006)

Follow-up Duration: 12 weeks (primary); ~2 years median in EAP Canada extension

Centers: 5

Countries: Canada

Sample Size: 20

Analysis: Descriptive; no formal hypothesis testing; 20 participants (10 per dose group) considered sufficient for TEAE rate estimation and PK characterization


Inclusion Criteria

  • Male sex
  • Age 2-<4 years
  • Duchenne muscular dystrophy confirmed by genetic testing and/or dystrophin deficiency on muscle biopsy
  • Evidence of varicella zoster virus immunity
  • Corticosteroid-naive (no prior oral glucocorticoid or oral immunosuppressive therapy)
  • Ambulatory at baseline
  • Written informed consent from parent/legal guardian

Exclusion Criteria

  • Prior or current oral glucocorticoid or immunosuppressive therapy
  • Immunosuppression
  • Diabetes mellitus
  • Major renal impairment
  • Major hepatic impairment

Baseline Characteristics

Characteristic2 mg/kg/d (N=10)6 mg/kg/d (N=10)
Median Age (Q1;Q3), y3.3 (3.0; 3.8)3.5 (3.3; 3.6)
Race - Asian50% (5/10)10% (1/10)
Race - Black or African American0%10% (1/10)
Race - White50% (5/10)80% (8/10)
Median Height z-score (Q1;Q3)-0.61 (-1.11; -0.31)-0.45 (-1.22; 0.54)
Median Weight z-score (Q1;Q3)0.16 (-0.62; 0.48)0.13 (-0.15; 1.03)
Median BMI z-score (Q1;Q3)0.54 (-0.24; 1.25)0.24 (-0.60; 1.55)
Median age at first symptoms (Q1;Q3), mo19.0 (18.0; 24.0)12.0 (12.0; 18.0)
Ambulatory100% (10/10)100% (10/10)
Median Bayley-III GMSS (Q1;Q3)3.5 (2.0; 4.0)5.0 (4.0; 6.0)

Arms

FieldVamorolone 2 mg/kg/dVamorolone 6 mg/kg/d
InterventionOral vamorolone suspension (4% w/v in flavored syrup) 2 mg/kg/d once daily at breakfastOral vamorolone suspension (4% w/v in flavored syrup) 6 mg/kg/d once daily at breakfast
Duration12 weeks + EAP extension12 weeks + EAP extension

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Safety and tolerability: TEAEs, SAEs, and change from baseline to week 12 in height, weight, and BMIPrimary2 mg/kg/d: 70% (7/10) had ≥1 TEAE (18 events); 0 SAEs; median CBL height-score -0.04, weight-score -0.01, BMI-score 0.126 mg/kg/d: 90% (9/10) had ≥1 TEAE (39 events); 0 SAEs; median CBL height-score 0.09, weight-score 0.13, BMI-score -0.13Not applicable (descriptive)
PK: Cmax day 1 (geometric mean)Secondary2 mg/kg/d: 246.8 ng/mL6 mg/kg/d: 781.7 ng/mLDescriptive
PK: Cmax week 2 (geometric mean)Secondary2 mg/kg/d: 349.3 ng/mL6 mg/kg/d: 719.5 ng/mLDescriptive
TmaxSecondary~2 h~2 hDescriptive
Adrenal suppression (cortisol ≤65 nmol/L) at week 12Secondary2 mg/kg/d: 20% (2/10)6 mg/kg/d: 40% (4/10)Descriptive
Bayley-III GMSS median change from baseline to week 12 (exploratory)Secondary2 mg/kg/d: 0.0 (Q1;Q3: 0.0; 1.0)6 mg/kg/d: 2.5 (Q1;Q3: 2.0; 4.0)Descriptive
Bone turnover (CTX1) median CBL, ng/LSecondary2 mg/kg/d: -35.56 mg/kg/d: 228.0Descriptive
Fasting glucose median CBL, mmol/LSecondary2 mg/kg/d: -0.1656 mg/kg/d: -0.385Descriptive
HbA1c median CBLSecondary2 mg/kg/d: 0.0016 mg/kg/d: 0.000Descriptive
Ease of administration (cooperatively without opposition)Secondary2 mg/kg/d: 9/106 mg/kg/d: 9/10Descriptive
Any TEAEAdverse70% (2 mg) vs 90% (6 mg)
Any drug-related TEAEAdverse10% (2 mg) vs 70% (6 mg)
Serious TEAEAdverse0% vs 0%
Discontinuations due to TEAEAdverse0% vs 0%
DeathsAdverse0 vs 0
GI disorders (SOC)Adverse10% (2 mg) vs 80% (6 mg)
DiarrheaAdverse0% vs 30%
Abdominal pain upperAdverse0% vs 20%
ToothacheAdverse0% vs 20%
Endocrine disorders (adrenal suppression)Adverse0% vs 50%
Infections and infestationsAdverse50% (2 mg) vs 30% (6 mg)
NasopharyngitisAdverse40% vs 30%
Musculoskeletal disordersAdverse0% vs 40%
Pain in extremityAdverse0% vs 20%
PyrexiaAdverse10% vs 10%
Psychiatric disorders (irritability, aggression, lip biting, mood swings)Adverse0% vs 20% (all mild, no treatment required)

Subgroup Analysis

Post hoc Bayley-III GMSS by age: patients ≤3.5 y had median CBL 0.5 (2 mg/kg/d, n=4) vs 2.0 (6 mg/kg/d, n=2); patients >3.5 y had 0.0 (n=5) vs 2.5 (n=8), showing dose-dependent motor improvement across age subgroups.


Criticisms

  • Small sample size (n=20; 10 per arm) limits precision of safety and efficacy estimates
  • Open-label, nonrandomized, single-country (Canada) design provides only Class IV evidence
  • Sequential (not concurrent) allocation to dose groups introduces potential temporal confounding
  • No placebo or classic corticosteroid comparator; efficacy interpretation relies on historical controls
  • Short 12-week primary treatment period is insufficient to fully evaluate long-term growth, bone health, or motor function; EAP follow-up used routine-care data quality only
  • Bayley-III is validated only up to 3.5 years, but was used in all participants; efficacy signal driven partly by patients above the validated age range
  • PK terminal half-life and AUC-infinity could not be calculated due to sparse sampling; high interpatient variability
  • Industry-sponsored study (Santhera/ReveraGen); several coauthors are company employees
  • Age distribution skewed toward the upper end of the 2-<4 y range (median 3.4 y), limiting generalizability to the youngest patients
  • Fasting insulin data unavailable for 2 mg/kg/d group due to central-laboratory sample-handling oversight

Funding

Santhera Pharmaceuticals (sponsor); vamorolone codeveloped with ReveraGen BioPharma

Based on: Vamorolone for DMD (Neurology, 2026)

Authors: Mah JK, Gonorazky HD, Nigro E, et al.

Citation: Neurology 2026;106(11):e218066

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