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ARAMIS Post hoc (LVO)

Dual Antiplatelet Versus Alteplase for Early Neurologic Deterioration in Minor Stroke With Versus Without Large Vessel Occlusion: Prespecified Post Hoc Analysis of the ARAMIS Trial

Year of Publication: 2024

Authors: Yu Cui, Chao He, Zi-Ang Li, ..., Hui-Sheng Chen

Journal: Stroke

Citation: Stroke. 2024;55:2590–2598

Link: https://clinicaltrials.gov/study/NCT03661411


Clinical Question

Is dual antiplatelet therapy more effective than intravenous alteplase in preventing early neurological deterioration in minor strokes without large vessel occlusion compared to those with large vessel occlusion?

Bottom Line

Among minor nondisabling acute ischemic stroke patients without large vessel occlusion, DAPT may be superior to intravenous alteplase in preventing early neurological deterioration with a better safety profile, but this benefit was not seen in patients with large vessel occlusion.

Major Points

  • Prespecified post hoc analysis of ARAMIS trial including 480 patients: 36 with LVO and 444 without LVO
  • DAPT significantly reduced early neurological deterioration (END) compared to alteplase in non-LVO group (0.5% vs 5.7%; adjusted RD -4.8%, 95% CI -6.9% to -2.6%, P<0.001)
  • No significant difference in END between treatments in LVO group (15.4% vs 13.0%; adjusted RD 2.3%, 95% CI -17.6% to 22.3%, P=0.82)
  • Marginally significant interaction between treatment effects and LVO status (Pint=0.06)
  • DAPT associated with significantly fewer bleeding events than alteplase in non-LVO group (0.5% vs 7.7%; adjusted RD -6.4%, 95% CI -8.9% to -3.9%, P<0.001)

Design

Study Type: Prespecified post hoc analysis of randomized controlled trial

Randomization: 1

Blinding: Open-label; NIHSS at baseline/24 h assessed by unblinded investigators; 90-day mRS and vascular events assessed by blinded assessors

Follow-up Duration: 90 days

Countries: China

Sample Size: 480

Analysis: As-treated analysis; generalized linear models with binomial distribution and identity link function for risk difference; ordinal regression for mRS shift; Cox regression for stroke/vascular events; adjusted for baseline imbalances (P<0.1); IBM SPSS 26.0 and R 4.1.0


Inclusion Criteria

  • Patients from ARAMIS trial as-treated analysis set
  • Age ≥18 years
  • Acute ischemic stroke with NIHSS ≤5 (minor) and nondisabling neurological deficit
  • Symptom onset within 4.5 hours
  • Responsible vessel examination available (CT angiography or MR angiography)

Exclusion Criteria

  • mRS scores before stroke ≥2
  • Definite indication for anticoagulation
  • History of intracerebral hemorrhage
  • No cerebral vessel examination available

Baseline Characteristics

CharacteristicControlActive
NoteAlteplase arm — arm-level baseline values reported in supplemental Table S2 (not in main text); qualitative arm imbalances summarized belowDAPT arm — arm-level baseline values reported in supplemental Table S2 (not in main text); qualitative arm imbalances summarized below
LVO group vs DAPTHigher blood pressure and higher NIHSS score at randomization
Non-LVO group vs DAPTLower mean age, lower proportion of hypertension, higher systolic BP, and higher NIHSS score at randomization
Adjusted covariates - LVO groupSystolic BP, diastolic BP, and NIHSS score at randomization
Adjusted covariates - non-LVO groupAge, history of hypertension, systolic BP, and NIHSS score at randomization
LVO group vs alteplaseLower blood pressure and lower NIHSS score at randomization
Non-LVO group vs alteplaseHigher mean age, higher proportion of hypertension, lower systolic BP, and lower NIHSS score at randomization
Overall LVO group (n=36) - Age62.4±9.4 y (Table 1)
Overall LVO group - Female16.7%
Overall LVO group - Current smoker52.8%
Overall LVO group - Hypertension47.2%
Overall LVO group - Systolic BP149.4±18.8 mm Hg
Overall LVO group - NIHSS2.0±1.2
Overall LVO group - OTT (min)178.6±55.2
Overall LVO group - Large artery atherosclerosis100%
Overall non-LVO group (n=444) - Age63.2±10.7 y (Table 1)
Overall non-LVO group - Female32.0%
Overall non-LVO group - Current smoker33.1%
Overall non-LVO group - Hypertension55.0%
Overall non-LVO group - Systolic BP151.9±16.9 mm Hg
Overall non-LVO group - NIHSS2.2±1.4
Overall non-LVO group - OTT (min)174.2±58.3
Overall non-LVO group - Small artery occlusion37.2%

Arms

FieldDAPTControl
InterventionClopidogrel 300 mg loading dose on day 1 followed by 75 mg daily for 12±2 days plus aspirin 100 mg daily for 12±2 days, then single or dual antiplatelets per guidelines until 90 daysIntravenous alteplase 0.9 mg/kg (maximum 90 mg) followed by guideline-based antiplatelet treatment starting 24 hours post-thrombolysis (DAPT for 12±2 days consistent with DAPT group)
Duration12±2 days initial DAPT, then guideline-based therapy to 90 daysSingle dose alteplase, then antiplatelet therapy

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Early neurological deterioration (END) at 24 hours, defined as ≥4-point NIHSS score increase compared with baseline, but not as a result of cerebral hemorrhagePrimaryLVO: 3/23 (13.0%); Non-LVO: 14/247 (5.7%)LVO: 2/13 (15.4%); Non-LVO: 1/197 (0.5%)LVO: 0.82; Non-LVO: <0.001
Excellent functional outcome at 90 days (mRS 0-1)SecondaryLVO: 20/23 (87.0%); Non-LVO: 228/246 (92.7%)LVO: 12/13 (92.3%); Non-LVO: 186/196 (94.9%)Adjusted RD — LVO: 9.4% (-8.2 to 27.1); Non-LVO: 1.3% (-1.9 to 4.5)LVO: 0.29; Non-LVO: 0.43
Favorable functional outcome at 90 days (mRS 0-2)SecondaryLVO: 21/23 (91.3%); Non-LVO: 237/246 (96.3%)LVO: 12/13 (92.3%); Non-LVO: 190/196 (96.9%)Adjusted RD — LVO: 6.4% (-10.6 to 23.5); Non-LVO: 0.3% (-2.1 to 2.7)LVO: 0.46; Non-LVO: 0.80
mRS distribution (shift) at 90 daysSecondaryAlteplase (LVO n=23; Non-LVO n=246)DAPT (LVO n=13; Non-LVO n=196)Adjusted OR — LVO: 2.16 (0.76 to 6.08); Non-LVO: 0.86 (0.63 to 1.16)LVO: 0.15; Non-LVO: 0.33
Early neurological improvement at 24 hours (≥2-point NIHSS decrease)SecondaryLVO: 4/23 (17.4%); Non-LVO: 48/247 (19.4%)LVO: 1/13 (7.7%); Non-LVO: 26/197 (13.2%)Adjusted RD — LVO: -6.4% (-23.5 to 10.6); Non-LVO: 0.5% (-4.4 to 5.3)LVO: 0.46; Non-LVO: 0.85
Change in NIHSS score at 24 hours (log-transformed)SecondaryLVO: median 0.00 (0.00 to 0.00); Non-LVO: median 0.00 (-0.69 to 0.00)LVO: median 0.00 (0.00 to 0.00); Non-LVO: median 0.00 (-0.41 to 0.00)Adjusted geometric mean ratio — LVO: 0.02 (-0.31 to 0.35); Non-LVO: 0.04 (-0.03 to 0.10)LVO: 0.90; Non-LVO: 0.25
Stroke or other vascular events within 90 daysSecondaryLVO: 0/23 (0.0%); Non-LVO: 1/246 (0.4%)LVO: 0/13 (0.0%); Non-LVO: 0/196 (0.0%)Adjusted HR — LVO: NA; Non-LVO: 3173.24 (0.00 to NA)LVO: NA; Non-LVO: 0.98
All-cause mortality at 90 daysSecondaryLVO: 0/23 (0.0%); Non-LVO: 0/246 (0.0%)LVO: 0/13 (0.0%); Non-LVO: 1/196 (0.5%)Adjusted RD — LVO: NA; Non-LVO: 0.4% (NA to NA)LVO: NA; Non-LVO: 0.47
Symptomatic intracranial hemorrhageAdverseLVO: 0/23 (0.0%); Non-LVO: 2/247 (0.8%)LVO: 0/13 (0.0%); Non-LVO: 0/197 (0.0%)Adjusted RD — LVO: NA; Non-LVO: -0.8% (NA to NA)LVO: NA; Non-LVO: 0.25
Bleeding eventsAdverseLVO: 0/23 (0.0%); Non-LVO: 19/247 (7.7%)LVO: 0/13 (0.0%); Non-LVO: 1/197 (0.5%)Adjusted RD — LVO: NA; Non-LVO: -6.4% (-8.9 to -3.9)LVO: NA; Non-LVO: <0.001

Subgroup Analysis

No significant interaction between treatment effects and groups regarding primary outcome in prespecified subgroup analysis by age, sex, history of diabetes, NIHSS score, time from onset to treatment, and location of responsible vessel


Criticisms

  • Unbalanced sample size between groups, especially small LVO group (n=36) affecting statistical power
  • 33.6% of patients excluded from as-treated analysis due to lack of vessel examination
  • 20.4% crossover rate in original ARAMIS trial introduces potential selection bias
  • Post hoc analysis nature limits strength of conclusions and requires confirmation
  • NIHSS assessment at 24 hours not blinded to treatment allocation introducing potential observer bias
  • Results may not be generalizable outside Chinese population
  • Did not investigate degree of vessel stenosis after treatment to confirm mechanistic hypothesis

Funding

Science and Technology Project Plan of Liaoning Province (2022JH2/101500020); funders had no role in study design, data collection, analysis, or interpretation

Based on: ARAMIS Post hoc (LVO) (Stroke, 2024)

Authors: Yu Cui, Chao He, Zi-Ang Li, ..., Hui-Sheng Chen

Citation: Stroke. 2024;55:2590–2598

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