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BUTCH

Effect of DL-3-n-Butylphthalide on Cerebral Hypoperfusion Due to Atherosclerotic Stenosis: A Multicenter, Double-Blind, Randomized Controlled, Preliminary Trial

Year of Publication: 2026

Authors: Chen D, et al.

Journal: CNS Drugs

Citation: CNS Drugs. 2026 Jul (epub 2026 Apr 30). doi:10.1007/s40263-026-01293-w. PMID: 42062656; PMCID: PMC13303579.

Link: https://doi.org/10.1007/s40263-026-01293-w

Bottom Line

In this preliminary multicenter RCT, NBP 600 mg/day for 4 weeks significantly increased the proportion of patients with cerebral blood flow amelioration at 12 weeks (adjusted RR 1.32, p=0.006) without an increase in adverse events, supporting NBP as a candidate medical therapy for chronic cerebral hypoperfusion in patients ineligible for or declining revascularization.

Major Points

  • Primary endpoint (CBF amelioration ≥10% on CTP at 12 weeks) was met: 55.4% NBP vs 43.9% placebo; adjusted RR 1.32 (95% CI 1.08–1.61), p=0.006.
  • Secondary perfusion measures (CBV, MTT, TTP amelioration; continuous perfusion changes) did not show significant differences between groups.
  • Clinical cerebral ischemic events were rare and similar between arms (any territory: 1.23% NBP vs 2.30% placebo, p=0.521).
  • Safety was comparable: total AEs 6.1% vs 5.0% (p=0.575); serious AEs 0.8% vs 0.8% (p=0.990).
  • Authors characterize this as a preliminary trial; a larger confirmatory study is registered (ChiCTR2500104739).
  • Population was almost entirely Han Chinese, enrolled across 38 Chinese centers, limiting generalizability.

Design

Study Type: Multicenter, double-blind, randomized, placebo-controlled preliminary trial

Randomization: 1

Blinding: Double-blind (patients, investigators, and drug administrators blinded)

Enrollment Period: January 14, 2022 – April 11, 2024

Follow-up Duration: 12 weeks (with 4 weeks of active treatment)

Centers: 38

Countries: China

Sample Size: 485

Analysis: Comparison of completers with CTP at 12 weeks (n=416); sensitivity analyses with multiple imputation


Inclusion Criteria

  • Age 35–85 years
  • ≥70% stenosis or occlusion in unilateral internal carotid artery (ICA) or M1 segment of middle cerebral artery (MCA)
  • Documented cerebral hypoperfusion in the ipsilateral MCA territory on imaging
  • No transient ischemic attack or ischemic stroke within the previous 2 weeks
  • Signed informed consent from patient or surrogate

Exclusion Criteria

  • ≥50% stenosis or occlusion in the contralateral ICA or M1 MCA
  • ≥50% stenosis or occlusion in bilateral carotid arteries or innominate artery
  • Patient preference for carotid artery stenting, carotid endarterectomy, or other revascularization
  • ≥10 mm infarction in both MCA territories on CT or MRI
  • Other cerebral diseases affecting perfusion (infection, degeneration, demyelination, tumor, trauma)
  • Use of NBP or vasodilatation therapy within the prior 30 days
  • Pregnant or lactating women
  • Severe cardiac, pulmonary, alimentary, or neoplastic disease; life expectancy ≤6 months
  • Serum creatinine ≥140 µmol/L or transaminases ≥3× upper limit of normal
  • Cerebral stenosis from non-atherosclerotic causes (dissection, vasculitis, moyamoya)
  • Allergy to NBP or to Apium graveolens
  • Participation in another clinical trial within the prior 3 months
  • Other situations deemed unsuitable for inclusion by investigators

Baseline Characteristics

CharacteristicControlActive
N241244
Median Age (years, IQR)65 (58–71)62 (55–70)
Sex - Male70.1% (169/241)63.1% (154/244)
Sex - Female29.9% (72/241)36.9% (90/244)
Han Nationality97.1%98.0%
Median SBP (mmHg, IQR)138.0 (128.0–148.5)135.0 (126.0–149.5)
Median DBP (mmHg, IQR)80.0 (76.0–89.0)80.0 (76.0–90.0)
Overweight/Obesity (BMI ≥24)59.7%61.1%
Hypertension66.0%73.0%
Diabetes36.5%36.9%
Hypercholesterolemia32.0%29.9%
Coronary Heart Disease16.6%18.9%
Prior Cerebral Ischemic Events62.2%56.2%
Current Smoker37.8%36.1%
Antithrombotic Therapy69.7%70.5%
Statin Therapy68.1%70.9%
Stenotic Location - ICA33.3%27.0%
Stenotic Location - MCA (M1)66.7%73.0%
Severe Stenosis (70-99%)58.9%62.7%
Occlusion (100%)41.1%37.3%
Baseline rCBF % (median, IQR)76.1 (66.3–82.8)74.7 (64.5–83.2)
Baseline rCBV % (median, IQR)90.2 (80.0–100.7)89.7 (79.5–99.8)
Baseline rMTT % (median, IQR)83.5 (70.4–92.9)83.9 (67.1–92.3)
Baseline rTTP % (median, IQR)90.4 (80.1–96.8)91.0 (77.8–97.0)

Arms

FieldDL-3-n-Butylphthalide (NBP)Control
InterventionNBP 200 mg orally three times daily (600 mg/day)Matching placebo 20 mg orally three times daily (60 mg/day, designed as no-effect level)
Duration4 weeks of treatment; imaging follow-up at 12 weeks4 weeks of treatment; imaging follow-up at 12 weeks

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Proportion of patients achieving cerebral blood flow (CBF) amelioration on CT perfusion at 12 weeks (defined as rCBF_after/rCBF_before − 1 ≥ 10%)Primary43.9% (93/212)55.4% (113/204)RR 1.32 (adjusted); RR 1.26 (unadjusted)0.006 (adjusted); 0.020 (unadjusted)
CBV amelioration at 12 weeks (≥10%)Secondary40.6% (86/212)44.6% (91/204)RR 1.10 (adj 1.19)0.255 (adj 0.418)
MTT amelioration at 12 weeks (≥10%)Secondary35.9% (76/212)33.3% (68/204)RR 0.93 (adj 0.99)0.572 (adj 0.583)
TTP amelioration at 12 weeks (≥10%)Secondary17.5% (37/212)21.1% (43/204)RR 1.20 (adj 1.22)0.793 (adj 0.917)
Median rCBF change (%)Secondary5.6 (−7.7 to 19.7)9.9 (−0.6 to 20.7)Median diff 3.7 (−0.1 to 7.4)0.059
Median rCBV change (%)Secondary4.9 (−9.1 to 16.6)6.9 (−3.9 to 20.0)Median diff 2.9 (−1.0 to 7.1)0.141
Median rMTT change (%)Secondary1.8 (−8.3 to 12.6)1.5 (−8.2 to 11.7)Median diff −0.1 (−3.3 to 3.3)0.980
Median rTTP change (%)Secondary0.2 (−3.5 to 5.1)1.5 (−2.3 to 5.5)Median diff 0.7 (−0.7 to 2.1)0.335
Cerebral ischemic event in stenotic territorySecondary1.24% (3/241)1.23% (3/244)RR 1.04 (0.21–5.11)0.959
Cerebral ischemic event in any territorySecondary2.30% (5/241)1.23% (3/244)RR 0.63 (0.15–2.60)0.521
Total Adverse EventsAdverse6.1% (15/244) NBP vs 5.0% (12/241) placebo; RR 1.24 (0.59–2.58), p=0.575
Serious Adverse EventsAdverse0.8% (2/244) vs 0.8% (2/241); RR 0.99 (0.14–6.96), p=0.990
Cerebral Ischemic Events (any territory)Adverse1.23% vs 2.30%; RR 0.63 (0.15–2.60), p=0.521
Cerebral Ischemic Events (stenotic territory)Adverse1.23% vs 1.24%; RR 1.04 (0.21–5.11), p=0.959

Subgroup Analysis

Sensitivity analyses using multiple imputation for missing CTP follow-up data confirmed the primary result; detailed prespecified subgroups (e.g., ICA vs MCA, severe stenosis vs occlusion) were not reported as formal interaction analyses.


Criticisms

  • Self-described as a preliminary trial; sample size modest with ~14% loss to imaging follow-up (485 randomized but only 416 had 12-week CTP).
  • Population almost exclusively Han Chinese across 38 Chinese centers, limiting generalizability to other ethnic groups and healthcare settings.
  • Short treatment course (only 4 weeks of active drug) with surrogate imaging endpoint (CT perfusion CBF amelioration) at 12 weeks; clinical outcomes (recurrent ischemia, cognition, function) were not powered endpoints.
  • Only the primary perfusion measure (CBF) was significant; other perfusion parameters (CBV, MTT, TTP) showed no difference, raising questions about mechanism and robustness.
  • Placebo arm received a low (20 mg TID) NBP dose described as a 'no-effect level' rather than a true placebo, which is unusual and may have biased the comparison.
  • Patients eligible for revascularization (CAS/CEA) were excluded, so results do not apply to typical surgical/endovascular candidates.
  • Funding from a single PLA institution and no industry sponsorship listed, but external validity for non-Chinese populations and longer-term safety remain unclear.

Funding

Clinical Research Project of Air Force Medical Center, Chinese PLA (No. 2021LC002). Trial registration: ChiCTR2100053112. Authors declared no conflicts of interest.

Based on: BUTCH (CNS Drugs, 2026)

Authors: Chen D, et al.

Citation: CNS Drugs. 2026 Jul (epub 2026 Apr 30). doi:10.1007/s40263-026-01293-w. PMID: 42062656; PMCID: PMC13303579.

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