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POST-TNK

Intra-Arterial Tenecteplase Following Endovascular Reperfusion for Large Vessel Occlusion Acute Ischemic Stroke: The POST-TNK Randomized Clinical Trial

Year of Publication: 2025

Authors: Jiacheng Huang, Jie Yang, Chang Liu, ..., Wenjie Zi

Journal: JAMA

Citation: JAMA. 2025;333(7):579-588. doi:10.1001/jama.2024.23466

Link: https://jamanetwork.com/journals/jama/fullarticle/2814102


Clinical Question

Does adjunct intra-arterial tenecteplase after successful thrombectomy improve functional outcome in acute LVO stroke?

Bottom Line

Intra-arterial tenecteplase after EVT did not significantly improve disability-free survival at 90 days and increased radiologic hemorrhage without increasing symptomatic ICH or mortality.

Major Points

  • Largest trial of adjunct intra-arterial tenecteplase post-EVT (n=540)
  • Included only patients with eTICI 2c–3 reperfusion and no prior IV tPA
  • 49.1% vs 44.1% had mRS 0–1 at 90 days (not statistically significant)
  • Symptomatic ICH rates similar (6.3% vs 4.4%), but radiologic ICH higher in tenecteplase group
  • No significant mortality difference (16.0% vs 19.3%)

Design

Study Type: Randomized, open-label, blinded outcome trial

Randomization: 1

Blinding: Outcome assessors were blinded; treating physicians were not

Enrollment Period: October 26, 2022 – March 1, 2024

Follow-up Duration: 90 days

Centers: 34

Countries: China

Sample Size: 540

Analysis: Intention-to-treat using modified Poisson regression, Cox models, and adjusted generalized odds ratio


Inclusion Criteria

  • Age ≥18 years
  • NIHSS ≤25
  • Anterior circulation LVO (ICA, M1, M2)
  • eTICI 2c–3 post-EVT
  • ASPECTS ≥6 within 6h or DEFUSE 3/DAWN eligible (6–24h window)
  • No prior IV thrombolysis

Exclusion Criteria

  • Prestroke mRS ≥2
  • Prior IV tPA
  • Hemorrhage on imaging
  • Procedure time >90 min
  • Life expectancy <90 days
  • Recent GI bleeding

Arms

FieldIntra-arterial tenecteplaseControl
InterventionTenecteplase 0.0625 mg/kg via catheter after EVTNo intra-arterial thrombolysis after EVT
DurationSingle dose post-reperfusionSingle EVT procedure

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
mRS 0–1 at 90 daysPrimary44.1%49.1%0.11
mRS 0–2 at 90 daysSecondary58.9%61.3%0.38
Death at 90 daysSecondary19.3%16.0%0.750.16
Symptomatic Intracranial HemorrhageAdverse4.4%6.3%1.430.35
Any Intracranial HemorrhageAdverse27.3%36.6%1.330.02

Subgroup Analysis

No interaction found across predefined subgroups (age, sex, NIHSS, ASPECTS, occlusion site, reperfusion grade)


Criticisms

  • No benefit in primary or secondary outcomes
  • Higher rate of radiologic hemorrhage
  • Excludes patients who received IV tPA, limiting generalizability
  • No post-procedure imaging to assess perfusion effects
  • Study conducted only in Chinese population

Funding

National Natural Science Foundation of China; China Postdoctoral Science Foundation; study drug provided by Shijiazhuang Recomgen Pharmaceutical

Based on: POST-TNK (JAMA, 2025)

Authors: Jiacheng Huang, Jie Yang, Chang Liu, ..., Wenjie Zi

Citation: JAMA. 2025;333(7):579-588. doi:10.1001/jama.2024.23466

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