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MUSETTE & GAVOTTE

Efficacy and safety of a bodyweight–adjusted higher dose of ocrelizumab in relapsing (MUSETTE) and primary progressive (GAVOTTE) multiple sclerosis: two multicentre, randomised, double-blind, parallel-group phase 3b trials

Year of Publication: 2026

Authors: Hauser SL, Giovannoni G, Montalban X, ..., on behalf of the MUSETTE and GAVOTTE Study Groups

Journal: Lancet

Citation: Lancet 2026; 407: 2180–94


Clinical Question

Does a bodyweight-adjusted higher dose of ocrelizumab provide better disability progression control than the approved 600 mg dose in relapsing or primary progressive multiple sclerosis?

Bottom Line

In both RMS (MUSETTE) and PPMS (GAVOTTE), bodyweight-adjusted high-dose ocrelizumab (1200/1800 mg) did NOT further improve control of disability progression compared with the approved 600 mg dose, despite greater peripheral B-cell depletion. Safety was comparable. The standard 600 mg dose remains the appropriate choice for both RMS and PPMS.

Major Points

  • Neither MUSETTE (RMS) nor GAVOTTE (PPMS) met its primary endpoint of superiority over the 600 mg dose
  • MUSETTE 12-week cCDP: 34% vs 37% (HR 0.93, 95% CI 0.73–1.18; p=0.53)
  • GAVOTTE 12-week cCDP: 47% vs 49% (HR 0.95, 95% CI 0.76–1.18; p=0.64)
  • Greater peripheral B-cell depletion with the high dose did NOT translate into improved clinical outcomes
  • Safety profiles were similar between dose groups; no new safety concerns identified
  • MUSETTE showed the lowest annualised relapse rate ever observed in a controlled phase 3 RMS trial
  • Findings suggest disability accumulation is driven largely by progression independent of relapse activity (PIRA) and may require targeting CNS-compartmentalised B cells or non-B-cell mechanisms
  • Circulating B-cell concentration alone may be an insufficient measure to guide ocrelizumab treatment decisions

Design

Study Type: Phase 3b, multicentre, randomised, double-blind, parallel-group, active-controlled superiority trials

Randomization: 1

Blinding: Double-blind (patients, investigators, and sponsor blinded to treatment allocation)

Allocation: 2:1 to high-dose ocrelizumab vs 600 mg ocrelizumab via permuted-block randomisation (block size 6) using IxRS; stratified by weight (<75 kg vs ≥75 kg), region (USA vs rest of world), age (≤45 vs >45 years); MUSETTE additionally by EDSS (<4 vs ≥4); GAVOTTE additionally by sex

Enrollment Period: MUSETTE: Nov 26, 2020 – Aug 30, 2022; GAVOTTE: Dec 3, 2020 – May 15, 2023

Follow-up Duration: MUSETTE: median 184.4 weeks; GAVOTTE: median 174.1 weeks (event-driven, minimum 120 weeks)

Centers: 271

Countries: MUSETTE: 21 countries, GAVOTTE: 22 countries

Sample Size: 1613

Analyzed: 1613

Analysis: Efficacy analysed in full analysis set (all randomised participants); safety analysed in all participants who received ≥1 infusion

Power Calculation: Event-driven design: MUSETTE required 205 cCDP events; GAVOTTE required 357 cCDP events

Registration: MUSETTE: NCT04544436; GAVOTTE: NCT04548999


Inclusion Criteria

  • Age 18–55 years
  • MUSETTE: Diagnosis of RMS (RRMS or active SPMS per 2017 revised McDonald criteria)
  • MUSETTE: EDSS score 0.0–5.5 at screening and baseline
  • MUSETTE: ≥2 relapses in the 2 years before screening, or 1 relapse in the year before
  • GAVOTTE: Diagnosis of PPMS per 2017 McDonald criteria
  • GAVOTTE: EDSS score 3.0–6.5

Exclusion Criteria

  • Previous use of anti-CD20 therapy in the 2 years before randomisation

Baseline Characteristics

CharacteristicMUSETTE: High-dose ocrelizumab (n=577)MUSETTE: 600 mg ocrelizumab (n=283)GAVOTTE: High-dose ocrelizumab (n=500)GAVOTTE: 600 mg ocrelizumab (n=253)
Age, years (median, range)35 (18–55)36 (18–54)43 (18–56)43 (18–56)
Female360 (62%)180 (64%)266 (53%)133 (53%)
White511 (89%)249 (88%)444 (89%)215 (85%)
Hispanic or Latino101 (18%)57 (20%)77 (16%)34 (14%)
BMI, kg/m²24.224.424.724.0
Weight, kg (median)70.070.672.172.0
Weight ≥75 kg224 (39%)108 (38%)216 (43%)105 (42%)
Baseline EDSS (median, range)3.0 (0.0–6.0)2.5 (0.0–5.5)4.5 (1.0–7.0)4.5 (3.0–6.5)
Disease duration, years5.65.85.75.3
Previous DMT use294 (51%)140 (49%)79 (16%)38 (15%)
≥1 T1 Gd-enhancing lesion225 (39%)110 (39%)131 (26%)66 (26%)
T2 lesions (median)46.043.055.057.0

Arms

FieldMUSETTE: High-dose ocrelizumabControlGAVOTTE: High-dose ocrelizumabControl
N577283500253
InterventionOcrelizumab 1200 mg (if baseline body weight <75 kg) or 1800 mg (if ≥75 kg) IV every 24 weeks; first dose split as two infusions 14 days apartOcrelizumab 600 mg IV every 24 weeks (approved dose); first dose split as two 300 mg infusions 14 days apartOcrelizumab 1200 mg (if baseline body weight <75 kg) or 1800 mg (if ≥75 kg) IV every 24 weeks; first dose split as two infusions 14 days apartOcrelizumab 600 mg IV every 24 weeks (approved dose); first dose split as two 300 mg infusions 14 days apart
DurationMinimum 120 weeks until 205 cCDP events accrued; median 184.4 weeksMinimum 120 weeks until 205 cCDP events accrued; median 184.4 weeksMinimum 120 weeks until 357 cCDP events accrued; median 174.1 weeksMinimum 120 weeks until 357 cCDP events accrued; median 174.1 weeks

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Time to onset of 12-week composite confirmed disability progression (cCDP), defined as first occurrence of: increase in EDSS ≥1.0 point from baseline (if baseline ≤5.5) or ≥0.5 points (if baseline >5.5); OR ≥20% increase in T25FWT from baseline; OR ≥20% increase in 9HPT from baselinePrimaryMUSETTE: 37% (104/283) with 600 mg; GAVOTTE: 49% (124/253) with 600 mgMUSETTE: 34% (198/577) with high-dose; GAVOTTE: 47% (235/500) with high-dose0.93MUSETTE p=0.53; GAVOTTE p=0.64
MUSETTE — 24-week cCDPSecondary29% (83/283)27% (155/577)0.92 (0.70–1.20)0.53
MUSETTE — 12-week cPIRASecondary34% (96/283)30% (173/577)0.87 (0.67–1.11)0.26
MUSETTE — ≥20% increase in T25FWT (12-wk)Secondary28% (79/280)25% (139/567)0.86 (0.65–1.13)0.27
MUSETTE — 12-week CDP-EDSSSecondary16% (45/283)17% (99/577)1.10 (0.77–1.57)0.59
MUSETTE — SDMT 4-point worsening (12-wk)Secondary15% (43/281)14% (81/568)0.88 (0.60–1.28)0.49
MUSETTE — MSWS-12 ≥8-point increase (24-wk)Secondary31% (74/240)25% (123/491)0.80 (0.60–1.07)0.13
MUSETTE — NfL % change from baseline (wk 48)Secondary−37%−37%<0.0001 (both arms)
MUSETTE — Annual % change in total brain volumeSecondary−0.397−0.402diff −0.0050.87
GAVOTTE — 24-week cCDPSecondary43% (109/253)40% (202/500)0.91 (0.72–1.15)0.41
GAVOTTE — 12-week cPIRASecondary48% (122/253)46% (229/500)0.94 (0.75–1.17)0.58
GAVOTTE — ≥20% increase in T25FWT (12-wk)Secondary42% (105/251)39% (191/494)0.93 (0.73–1.18)0.55
GAVOTTE — 12-week CDP-EDSSSecondary30% (75/253)26% (130/500)0.86 (0.65–1.15)0.32
GAVOTTE — SDMT 4-point worsening (12-wk)Secondary19% (47/249)17% (85/487)0.92 (0.64–1.32)0.65
GAVOTTE — MSWS-12 ≥8-point increase (24-wk)Secondary42% (85/201)46% (176/382)1.22 (0.94–1.59)0.13
GAVOTTE — Annual % change in thalamic volumeSecondary−0.974−1.006diff −0.0320.70
GAVOTTE — Annual % change in total brain volumeSecondary−0.480−0.548diff −0.0690.08
GAVOTTE — NfL % change from baseline (wk 96)Secondary−22%−18%<0.0001 (both arms)
High-dose ocrelizumab showed a safety profile consistent with the approved 600 mg dose in both trials; no new safety signals. Adverse-event columns: Control = 600 mg, Intervention = high-dose (1200/1800 mg by body weight).Safety
MUSETTE — Any adverse eventAdverse267 (94%)552 (96%)
MUSETTE — Serious adverse eventAdverse34 (12%)77 (13%)
MUSETTE — Fatal adverse event (deaths)Adverse1 (<1%)4 (1%)
MUSETTE — Serious infections (incl COVID-19)Adverse13 (5%)35 (6%)
MUSETTE — Infusion-related reactionAdverse79 (28%)198 (34%)
MUSETTE — COVID-19Adverse104 (37%)217 (38%)
MUSETTE — Withdrawal due to AEAdverse7 (2%)20 (3%)
MUSETTE — MalignanciesAdverse04 (1%)
GAVOTTE — Any adverse eventAdverse230 (91%)447 (90%)
GAVOTTE — Serious adverse eventAdverse29 (11%)61 (12%)
GAVOTTE — Fatal adverse event (deaths)Adverse3 (1%)2 (<1%)
GAVOTTE — Serious infections (incl COVID-19)Adverse17 (7%)22 (4%)
GAVOTTE — Infusion-related reactionAdverse59 (23%)120 (24%)
GAVOTTE — Withdrawal due to AEAdverse6 (2%)11 (2%)
GAVOTTE — MalignanciesAdverse4 (2%)4 (1%)

Criticisms

  • Despite achieving greater peripheral B-cell depletion, the high dose did not translate to improved clinical outcomes — suggesting circulating B-cell levels alone may not be an adequate biomarker for treatment optimization
  • Pre-existing favourable control of disease activity with the 600 mg dose may have limited the ceiling for incremental benefit (lowest ARR ever observed in a phase 3 RMS trial)
  • Protocol deviations were notable: 19% of MUSETTE and 24% of GAVOTTE participants had major protocol deviations, though balanced between arms
  • Trials cannot address whether targeting CNS-compartmentalised B cells (which may not be reflected by peripheral B-cell depletion) would yield benefit

Funding

F Hoffmann-La Roche

Based on: MUSETTE & GAVOTTE (Lancet, 2026)

Authors: Hauser SL, Giovannoni G, Montalban X, ..., on behalf of the MUSETTE and GAVOTTE Study Groups

Citation: Lancet 2026; 407: 2180–94

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