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CombiRx

Combination Therapy with Interferon Beta-1a and Glatiramer Acetate in Relapsing-Remitting Multiple Sclerosis

Year of Publication: 2013

Authors: Lublin FD, Cofield SS, Cutter GR, ..., Wolinsky JS; CombiRx Investigators

Journal: Annals of Neurology

Citation: Lublin FD et al. Ann Neurol. 2013;73(3):327-340. DOI: 10.1002/ana.23863

Link: https://pubmed.ncbi.nlm.nih.gov/23483994/


Clinical Question

Is combination therapy with interferon beta-1a plus glatiramer acetate superior to either drug alone for reducing relapse rate in relapsing-remitting multiple sclerosis?

Bottom Line

Combination therapy with IFN beta-1a and GA was NOT superior to GA monotherapy for reducing relapse rate in RRMS (ARR 0.22 vs 0.25, p=0.27). Unexpectedly, GA alone was significantly better than IFN alone (p=0.03). The combination did show MRI benefits over IFN alone but not over GA alone, while causing more adverse events. This landmark negative trial ended the longstanding question of whether combining these two first-line therapies would produce synergistic benefit.

Major Points

  • Primary endpoint negative: combination ARR 0.22 not significantly different from GA alone ARR 0.25 (p=0.27)
  • GA alone was significantly superior to IFN alone for relapse rate (0.25 vs 0.33, p=0.03) -- unexpected finding favoring GA
  • Combination was superior to IFN alone (0.22 vs 0.33, p=0.03) but this benefit was entirely attributable to the GA component
  • MRI: combination reduced new/enlarging T2 lesions vs IFN alone but not vs GA alone
  • No significant differences in disability progression (EDSS) among the three arms
  • Combination had highest adverse event burden without added efficacy over GA monotherapy
  • Largest NIH-funded MS treatment trial; definitively answered the combination therapy question

Design

Study Type: Phase 3, multicenter, randomized, double-blind, double-dummy, parallel-group trial

Randomization: 1

Blinding: Double-blind, double-dummy (all patients received both IM injection and SC injection, one active and one placebo)

Enrollment Period: 2005-2009

Follow-up Duration: 3 years

Centers: 70

Countries: United States

Sample Size: 1008

Analysis: Intention-to-treat; 2:1:1 randomization (combo:IFN:GA); NCT00211887


Inclusion Criteria

  • Age 18-60 years
  • Relapsing-remitting MS by McDonald criteria
  • EDSS score 0-5.5
  • At least 2 relapses in the 3 years prior to enrollment or at least 1 relapse in the 13 months prior
  • MRI showing lesions consistent with MS
  • Able to self-inject or have caregiver administer injections

Exclusion Criteria

  • Primary or secondary progressive MS
  • Prior treatment with IFN beta or GA for more than 6 months
  • Treatment with mitoxantrone, cyclophosphamide, or other immunosuppressants within 12 months
  • Treatment with natalizumab at any time
  • Significant medical illness (hepatic, renal, cardiac, pulmonary)
  • Pregnancy or planned pregnancy during study period
  • History of depression with suicidal ideation

Arms

FieldCombination (IFN + GA)Interferon beta-1a AloneGlatiramer Acetate Alone
InterventionInterferon beta-1a 30 mcg IM weekly + glatiramer acetate 20 mg SC dailyInterferon beta-1a 30 mcg IM weekly + SC placebo dailyIM placebo weekly + glatiramer acetate 20 mg SC daily
Duration3 years3 years3 years

Outcomes

OutcomeTypeControlInterventionHR / OR / RRP-value
Exacerbation (relapse) rate over 3 yearsPrimaryGA alone: ARR 0.25; IFN alone: ARR 0.33Combination: ARR 0.22Combo vs GA: p=0.27 (NS); Combo vs IFN: p=0.03; GA vs IFN: p=0.03
Secondary
Secondary
Secondary
Secondary
Secondary
Flu-like symptomsAdverseGA: 19%Combo: 53%, IFN: 49%
Injection-site reactions (SC)AdverseIFN: 7%Combo: 47%, GA: 50%
Liver enzyme elevationAdverseGA: 3%Combo: 14%, IFN: 12%
DepressionAdverseGA: 11%Combo: 15%, IFN: 17%
LeukopeniaAdverseGA: 2%Combo: 8%, IFN: 7%
Any serious adverse eventAdverseIFN: 16%, GA: 13%Combo: 18%

Subgroup Analysis

Results consistent across subgroups by baseline EDSS, prior relapse frequency, and MRI activity; no subgroup showed combination benefit over GA


Criticisms

  • 2:1:1 randomization (499 combo vs 250 IFN vs 259 GA) gives more statistical power for combo comparisons but less for head-to-head IFN vs GA
  • IFN beta-1a 30 mcg IM weekly (Avonex dose) is the lowest IFN dose; higher-dose IFN formulations (44 mcg SC) might have performed differently
  • 3-year duration may be insufficient to detect differences in disability progression
  • Double-dummy design required patients to receive both IM and SC injections, increasing placebo-related injection-site reactions
  • No dose optimization period: fixed IFN dose from start may increase early dropout due to flu-like symptoms
  • Trial did not include high-efficacy comparators (natalizumab, fingolimod) that were available by completion

Funding

National Institute of Neurological Disorders and Stroke (NINDS), NIH -- non-industry

Based on: CombiRx (Annals of Neurology, 2013)

Authors: Lublin FD, Cofield SS, Cutter GR, ..., Wolinsky JS; CombiRx Investigators

Citation: Lublin FD et al. Ann Neurol. 2013;73(3):327-340. DOI: 10.1002/ana.23863

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